Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDFVMKFWQRLFTAFLQRFHLMRFFGRNRSFKELHQSSYAQEISKNLSKRLLNASRAQTNDLLKQFD-THLMGLTEEQAYTQQMTVGLNEVTHEKPLTWWQHLWYCYRNPFNILLSLLALIAF----FTDDLTGST------IISVMVILSTLLRYWQEAKSNQAADALKAMVSNTATVLRHQVSAEDLELMHERYGIDTKNQTTHQFEISIQYLVPGDVILLSAGDMIPADCRILSAKD--LFVSQAAMTGESMPVEKFPLQKNLEETSALELD--NIVFMGTNIVSGSAQAVVLSTGIQTYFGALAHRVTATDRSTTAFQMGVNKVSWLLIRFMLVMAPVVLFIN------GFTKGDWAEAFLF----ALSVAVGLTPEMLPMIVTSTLAKGAVFLSKKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLSQHIDVQGEKSDFVLMQAFL---NSYYQTG--LKN-----------LLDVAVLEAV--DDQIKIQKLR--YKKLDEV-------------------------------------------PFDFDRRRMSVVVKTPQQKA-------HMITKGAVEEMLKVCRYVEVNGKVEPLTKQREVAIEALTQRY--NRDGLRVVAVAYREFKNHQENYSVVD-------ESDLILIGYITFLDPPKESAKEAVQSLHAHGVTVKVLTGDNEFVTQKVCREIGL------NYDQVLLGGVIETLTDQQLKRAVEQYHIFAKLSPVHKERIVEQLKVNGHVVGFLGDGINDAAAIRAADIGISVDTAVDIAKESADLILLEKSLMVLEKGVIEGRRTFANMLKYIKMTASSNFGNVFSVLIASAFIPFLPMLPIHLLIQNLLYD-VSQIVIPFDNVDEELIAKPQRWQPEEVGRFMVVFGPISSIFDMITFGVMWFVFSANTPEHQTLFQSGWFVVGLLTQTLIVHMIRTAQIPFIQSRAATPLLIMTAVIMCIGIFLPMGPLASYLKLQALPLSYFLYLPLILGAYICVTQWVKKIYIRRYGWQ 1IWO Chain:A ((4-826)) ------------------------------------------------------AHSKSTEECLAYFGVSETTGLTPDQVKRHLEKYGHNELPAEEGKSLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRA-----------DRKSVQ---------RIKARDIVPGDIVEVAVGDKVPADIRILSIKSTTLRVDQSILTGESVSVIKH--TEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVSTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDFCSLNEFSITGSTYAPEGEVLKNDKPIRSGQFDGLVELATICALCNDSSLDFNETKGVYEKVGEATETALTTLVEKMNVFNTEVRNLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPMTGPVKEKILSVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSSRFMEYETDLTFVGVVGMLDPPRKEVMGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPR-SPKE--------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1IWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3496 57195 16.36 80.33 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 16.36 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_1IWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IWO-query.scw PDB file : Tito_Scwrl_1IWO.pdb: