Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKLAILILSLAFFGSAHADVKTLEQNLKTNYPDLPVKGVYQSPVAGIYEVYTSGRIVYTNQDAKYFFVGNLVDIKQQK--NMTEERI-AELGKIDVKSLPLNQAIKYVKGKGERTLYIFSDPDCPYCQRLEQNMVGVD--NVTVYVFLYPLTSLHPNAEKVSNQIWCSKNPSEAWTNYMLNRKLPAAGKSCTSPVQKNIALGQKLNIDGTPTLFLQDGQKISGVPGDAKQIEELLQSVK 4ILF Chain:A ((22-198)) -------------------------------------------SPVAGMKTVLTHSGVLYVTDDGKHIIQGPMYDVSGAHPVNVTNKLLMSQLNALE------KEMIVYKAPDEKHVITVFTDITCGYCHKLHEEMKDYNALGITVRYLAFPAQGLESQAEQDMKSIWCAKDKNKAFDDAMAGKGVKPA--SCDVNIADHYALGVQLGVSGTPAIVLSNGYVVPGYQG-------------

General information: TITO was launched using: RESULT: Template: 4ILF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 788 4631 5.88 26.92 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 5.88 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_4ILF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ILF-query.scw PDB file : Tito_Scwrl_4ILF.pdb: