Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKATEYIDQMQNMIGNLVNKGAAY---PASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASERV--DVEVEKKHPFDFVLWKHAKENEPSWASPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLMFPHHENEIAQSEASTGE-QYVNYWIHVGFINVDGEKMSKSLGNFFTIRDV-MEKFHPEVIRYFIVSSHYRSPVNFSDVALKEAKTSLTRFYHSFKAYQQVYGQTTTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 3C8Z Chain:A ((23-381)) -----LFDSADRQVRPVTPGPTA--TMYVCGITPYDATHLGHAATYLTFDLVHRLWLDAGHTVQYVQNVTDVDDPLFERAERDGIDWRTLGDRETQLFREDMAALRVLPPHDYVAATDAIAEVVEMVEKLLASGAAYIVEDAEYPDVYFRADATAQFGYESGYDRDTMLTLFAERGGDPDRPGKSDQLDALLWRAERPGEPSWPSPFGRGRPGWHVECSAIALTRIGTGLDIQGGGSDLIFPHHEYSAAHAESVTGERRFARHYVHTGMIG-------------VLVSQLRAQGVDPSAIRLGLFSGHYREDRFWSNEVLDEANARLAR-WRSATALPEAPDAT-------DVIARVRQYLADDLDTPKALAAL----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C8Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1761 -43456 -24.68 -128.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -24.68 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_3C8Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C8Z-query.scw PDB file : Tito_Scwrl_3C8Z.pdb: