Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAIANNHVVSFHYKLTNAEGETLDQSQGEPLAYLHGAGNIIPGLENALTGKTVGDKFTVNVPAAEGYGEYNPDLVQEVPAQMFQGVDNIQPGMQFQAQTDDGVQI-VTVKAVEGDNVIVDANFPLAGQDLTFEVEIVEIREASQEELDHGHVHGAGGHHH 3CGN Chain:A ((3-143)) ---VGQDKVVTIRYTL-QVEGEVLDQGE---LSYLHGHRNLIPGLEEALEGREEGEAFQAHVPAEKAYGPHDPEGVQVVPLSAFPEDAEVVPGAQFYAQDMEGNPMPLTVVAVEGEEVTVDFNHPLAGKDLDFQVEVVKVREATPEEL-------------

General information: TITO was launched using: RESULT: Template: 3CGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 559 -31482 -56.32 -224.87 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -56.32 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_3CGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CGN-query.scw PDB file : Tito_Scwrl_3CGN.pdb: