Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVGTRHLIYVQHNNEYKVAKYGHWDGLDKNT---------FEVYQGFNKAPLDSSERFASITSPDSNEGYYQVKFLESFDLDNLPSEEDFIAQLEREKN 5KEV Chain:B ((50-109)) -IIIRELVKSENKNYYNVI--GHGDIIQKNTHQYYLNFDNIDVYTGTNK-----YKEPTSISS------------------------------------

General information: TITO was launched using: RESULT: Template: 5KEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 92 2687 29.20 58.40 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : 29.20 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_5KEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KEV-query.scw PDB file : Tito_Scwrl_5KEV.pdb: