Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENNLILPALILVPFIAGFTCWLVDKLDKTLPRWIALIGMLITLGLGLALWQSGTYSYELGSKVPTWTAEFYLPWIQSLGIGIHLAVDGLSLLMVLLTALLGVLAVGCSWGEIQKNVGFFHLNLLWSLGGVIGVFLAIDLFLFFFFWEMMLVPIYFLIALWGHKGSNGRSRVYAATKFFLYTQIAGLVMLIGILGLVVYGYMMTGMIGFDYNYLLAVANRLPAGFAYGFM-ICFFIGFAVKLPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRLLAYTSISHMGFVLLAIYAGNILTFQGLMIMMLAHGLSSAALFIMSGQIYERLHTRDLRLMGGLRGQLQYLPFFLMFFVAALIGVPGLGNFIGEFLILMGSFNKYPVFTIIASISLVFAGLYGLILIHRALFGEPNSEQKQHYTSPLKDLS---AREVSILMICAIGLVWLGIYPQTFLDVSHSSMQWLVNSYVPVQEFVETVQQTATQLDHVEIQ 5LC5 Chain:M ((106-437)) ----------------------------------------------------------------------------------------------------------------------------------LIMTFTAMELILFYILFEATLVPTLIIITRWG----NQTERLNAGLYFLFYTLAGSLPLLVALI------YIQNTVGSLNFLMLQYWVQPVHNSWSNVFMWLACMMAFMVKMPLYGLHLWLPKAHVEAPIAGSMVLAAVLLKLGGYGMLRITLILNPMTDF-MAYPFIMLSLWGMIMTSSICLRQTDLKSLIAYSSVSHMALVIVAILIQTPWSYMGATALMIAHGLTSSMLFCLANSNYERIHSRTMILARGLQTLLPLMATWWLLASLTNLALPPTINLIGELFVVMSTFSWSNITIILMGVNMVITALYSLYMLIMT--------QRGKYTYHINNISPSFTRENALM-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5LC5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 1512 -261381 -172.87 -796.89 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain M : 0.65 3D Compatibility (PKB) : -172.87 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5LC5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LC5-query.scw PDB file : Tito_Scwrl_5LC5.pdb: