Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKNCGSGQSKLKALRMHIYFEVDFQEQAQHYQAVLHSRGVTVDLQPIEKLNARFLRLNPDLALCVDENGLWLSANGMKM-QPDWKAEIPRLKRASLKSEMIARACQLGEKPVLVDATAGLGHDSLLMAYLGAQIQLVERHPILFTLLEDSKAQAQRDPFLSQFMDRIQLIFADS---ASYLKQLDQEEKTVDVVYLDPMFPQRDQNQQAIKKQAQVKKQMQLLHLLLPEDGEMDLGGHLLELAKKVAKRVIVKRPRHAIFLANQEPAHQWQGDACRFDAYFQ 3EGI Chain:A ((13-162)) ---------------------------------------------------------------------------DGIKLDREGWFSVTPE----KIAEHIAGRVSQSFKCDVVVDAFCGVGGNTIQFALTGMRVIAIDIDPVKIAL-------ARNNAEVYGIADKIEFICGDFLLLASFLK--------ADVVFLSPPW--------------ATAETFDIRTMMSPD------GFEIFRLSKKITNNIVYFLPRNA------------------------

General information: TITO was launched using: RESULT: Template: 3EGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 665 -25052 -37.67 -177.67 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -37.67 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_3EGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EGI-query.scw PDB file : Tito_Scwrl_3EGI.pdb: