Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDRIMQITIRGHHLAITPAIEENIKAKFNQLTKHLDQVNSMQIKLTKDHQIDKRSHKGSSNHVAEAIVRLPGIELFAQATADDMYTSIKKLTEKLKKQLLKYRKMQCTYSQVAVSI 1N3G Chain:A ((1-87)) -----MTMNITSKQMEITPAIRQHVADRLAKLEK-------WQTHLINPHIILSKEPQG---FVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNK---------------

General information: TITO was launched using: RESULT: Template: 1N3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 386 -36754 -95.22 -422.46 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -95.22 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_1N3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N3G-query.scw PDB file : Tito_Scwrl_1N3G.pdb: