Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDEEDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPY---TSDDGFSVVNYQAINDRLGTWEDIEEM-----RSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDTDTSSVTR------PRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLN------PVRGI--LPEKTILE--LVEQLKKEGAR-VNYKKNPDGTTSPYEINATYLDALSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRND---YKGVEKTGENRKINRQKFLLADIEKELQTDPLRTA--VYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEEKVLVIHNFS---NQSAGYLLPKGDY--LDLFSGQIIHCSQERKLIFQPYEYMWLKIIK 1G5A Chain:A ((130-627)) ---------------------------------------------------------------------------------LHLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIFPDQHPGGFSQLEDG---RWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVAFIWKQMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSEAIV-HPDQVVQY-IGQDECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFQYNP--STGDCRVSGTAAALVGLAQDDPH-AVDRIKLLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQ--RNDPSTAAGQIYQDLRHMIAVRQSNPRFDGGRLVTFNTNNKHIIGYIR---NNALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVSLNQD--LTLQPYQVMWLEI--

General information: TITO was launched using: RESULT: Template: 1G5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2443 25066 10.26 54.14 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 10.26 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_1G5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G5A-query.scw PDB file : Tito_Scwrl_1G5A.pdb: