Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGIVSNDRGK-LKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFKFHDQSIGLAHNGNLINARSLRKEL-EENGAIFHSNSDTEILMHLIRRSE---------ETDFLNKLKSALNE-VKGGFAYLLMT-ETAMIAALDPNGFRPLAIGQM----KNGAYVIASETCALEVIGARFIRDVQPGEIVIINDEGIQIDCFTKDTQLS-ICSMEYIYFARPDSNIAGINVHTARKNMGRNLAKE-----SPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELREQGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCMAYFNGDYPTPLYDYEEKYQESLKEKVSFF 1ECJ Chain:A ((1-478)) -----------CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVF-EARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVN-SPYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFA-SELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFEC-SVFNGVYVTK--DVDQGYLDFL-------

General information: TITO was launched using: RESULT: Template: 1ECJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2803 6437 2.30 14.15 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 2.30 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_1ECJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECJ-query.scw PDB file : Tito_Scwrl_1ECJ.pdb: