Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQESNTLIFDVPDAFQVESIIGQGAYGAVCKAV--FCDDQIAVKKIPHYSRSEDTARRVLREIEILQNLQFCEQVVGCRLFFRPK---SEEKDVYVAMDYIPADLSSVIKNG-AITLDESVVRYITCQLLLALRALHRCKVLHRDVSTRNILIHYNSQVFLCDFGLSRFF--DPDEQLSFG---VVTQWYRAPEIILD-AAYSYASDVWSVGVILGELLLRRHLFPGKSNDSANQLQLIFHLVGTPSKDVFDADRSFGRASQNAKNYALAYIERRPCPSTLVNLLSTAPVLHHSAIAAVPPQAVQLAEQLLQFDPAKRPSADEALRHPWFDPCRAFIDEVVQHQDEEEIPVFTQTQNMNVEELVKRIEEVVPVFSEDLLVEDDGGGAAGSA 4IC7 Chain:A ((49-349)) ------VTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKH-DNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEH--VRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGK--NYVHQLQLIMMVLGTPSPAVIQA-----VGAERVRAYIQSLPPRQPVPWETV-------------YPGADRQALSLLGRMLRFEPSARISAAAALRHPFL-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4IC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1342 -50718 -37.79 -175.49 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -37.79 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4IC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IC7-query.scw PDB file : Tito_Scwrl_4IC7.pdb: