Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CLGVLLRNDGRKPILGFEPFRIRRTVSFPGYDFVLKVGRRCITEKISGTIPPHLHVHVVWGFPAPVRPVCNVYNGWTERFERYGRNVREAPW 4M7P Chain:A ((257-291)) ---------------------VRAFGNLPGWHLVLQIGRK-VTPAELGELPDNVEVH-----------------------------------

General information: TITO was launched using: RESULT: Template: 4M7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 86 -17317 -201.35 -494.76 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -201.35 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_4M7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7P-query.scw PDB file : Tito_Scwrl_4M7P.pdb: