Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVAICKNASPVHCNYLKCTNLA--------AGFSAG--TSTDVLSSGTVGSIGNDPQAQRQ 4XEP Chain:A ((79-123)) ------------------------------------------MGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVIYSGTVAA----------

General information: TITO was launched using: RESULT: Template: 4XEP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 37 -1560 -42.15 -44.56 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -42.15 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_4XEP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XEP-query.scw PDB file : Tito_Scwrl_4XEP.pdb: