Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FVNTTESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHY 3EAC Chain:A ((99-156)) ----------------------PETGLFLVRESTNYPGDYTLCVS-CDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHY

General information: TITO was launched using: RESULT: Template: 3EAC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 176 -39521 -224.55 -681.39 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -224.55 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_3EAC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAC-query.scw PDB file : Tito_Scwrl_3EAC.pdb: