Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGYPLSQQLQPSAKQFHKLCNLGKYPIEE----RKKEDRLACERLQCDFRHLSYYECLYRKDRNGNFLYRHI----YSELKNEDTLKN--DIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGR-ALEFMRYKVIYYEDFPY-VSDSSMVSY-----MGKTKELKMYQEELDEKHYIDRISSILCYKSQILIIWKSVEKLLNNIKELYLRNG--AAYSIRFWIKK 2X9L Chain:A ((8-265)) ------TRLLAISPHLDDAVLSFGAGLAQAAQDGANVLVYTVFAGA-AQPPYSPAAQRMHTIWGLA--PDDDAVLYRRKEDIAALDHLRVAHRHGRFLDSIYRKLPDGRWLTA--AVNDHSPDSDHDLVGEVADDIRSIIDEFDPTLVVTC-AAIGEHPDHEATRDAALFATHEKNVPVRLWEDLPYAVFKSGAVELPQGFRLGSADVSSVKPEMRSQK-----FQAVERYSSQMVLLNG--NNLFDRLDEHARQNAPHGGYGETTW---

General information: TITO was launched using: RESULT: Template: 2X9L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1175 -12440 -10.59 -54.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -10.59 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2X9L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X9L-query.scw PDB file : Tito_Scwrl_2X9L.pdb: