Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVEQFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFL----FASKDSGAIL--KIAREKLGNDKVVKLPTLIQTISQKFKNLFAKK 4BNQ Chain:A ((57-166)) -AKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKL-------LNKLG-NTTDRR-AQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKLDKTMAQ---------

General information: TITO was launched using: RESULT: Template: 4BNQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 382 2692 7.05 25.88 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 7.05 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_4BNQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BNQ-query.scw PDB file : Tito_Scwrl_4BNQ.pdb: