Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPASMMMGMPFLAFLSVVAVGFFASR--------VAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLMRTTNDITQVQMLITMDLQVVTCGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTT---VLMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTR 5MKK Chain:A ((77-247)) ----------------------------------------------------LGFLAVRAVHFAATYGETYLIQWVGQRVLFDLRSDLFAKLMRLHPGFYDRNPVGRLMTRVTSDVDAINQFITGGLVGVIADLFTLVGLLGFMLFLSPKLTLVVLLVAPVLLAVTTWVRLGMRSAYREMRL---RLARVNAALQENLSGVETIQLFVKEREREEKFDRLNRDLFR

General information: TITO was launched using: RESULT: Template: 5MKK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 164 -18281 -111.47 -114.26 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -111.47 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_5MKK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MKK-query.scw PDB file : Tito_Scwrl_5MKK.pdb: