Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVFVCLGNICRSPMAEFVMKSM------TDNYEIQSRATSSWEHGNPIHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDL-RQMCPVD-CQDKIYSFSSES------VPDPWYTG--DFEETYRRVQEGCQAWLERLEKES 5PNT Chain:A ((6-155)) -KSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWRVDSAATSGYEIGNPPDYRGQSCMKRHGIPM--SHVARQITKEDFATFDYILCMDESNLRDLNRKSNQVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFLEK-----

General information: TITO was launched using: RESULT: Template: 5PNT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 626 6770 10.81 50.52 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 10.81 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_5PNT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5PNT-query.scw PDB file : Tito_Scwrl_5PNT.pdb: