Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLGNVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEIGDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAEPVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQD---------------VPE-NVVVAG-VPAR----------IIKEIDAQTQQKTALEDALRTL 3GOS Chain:A ((106-275)) ----------------------------------------------------------------------------------------RVVPPATVRKGAFIARNTV-LMPSYVNIGAFVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFVGARSEVVEGVIVEEGSVISMGVFIGQSTRIYDRETGEVHYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDA------GINELLRTI

General information: TITO was launched using: RESULT: Template: 3GOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 788 -110988 -140.85 -810.13 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -140.85 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_3GOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GOS-query.scw PDB file : Tito_Scwrl_3GOS.pdb: