Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEFKFTDLVEPVAVDKKTALRITVLGGGSFGTAMANLAARNGCDTMIWIRDAETAEEINKTHINKRYLPDFTLESSLRAVSDLEQAVCDRDIILVAIPSHSFRDVLKQIAPYITAQA------VVSLTKGIEAKTFSFMSDIIREELPEVPYGVLSGPNLAKEIMAGMPSGTVIASDSELVRYAVQHALHSA--LFRVFGSDDVHGVELGGALKNIYAVAMGIGAAYKIGENTKSMILTRALAEMSRFAVKQGANPLTFLGLSGVGDLFATCNSPLSRNYQIGYALGSGKTLEQASKELGQTAEGINTIVQVRGKAQELDVYMPITNALYEVIFEGAPPLNIALSLMKNGHRSDVEFVLPHHEV 1JDJ Chain:A ((17-350)) ---------------------KAVVFGSGAFGTALAMVLSKKCREVCVWHMNEEEVRLVNEKRENVLFLKGVQLASNITFTSDVEKAYNGAEIILFVIPTQFLRGFFEKSGGNLIAYAKEKQVPVLVCTKGIERSTLKFPAEIIGEFLPSPLLSVLAGPSFAIEVATGVFTCVSIASADINVARRLQRIMSTGDRSFVCWATTDTVGCEVASAVKNVLAIGSGVANGLGMGLNARAALIMRGLLEIRDLTAALGGDGSAVFGLAGLGDLQLTCSSELSRNFTVGKKLGKGLPIEEIQR---AVAEGVATADPLMRLAKQLKVKMPLCHQIYEIVYKKKNPRDALADLLSCGLQDE----------

General information: TITO was launched using: RESULT: Template: 1JDJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1954 -98853 -50.59 -306.05 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -50.59 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1JDJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JDJ-query.scw PDB file : Tito_Scwrl_1JDJ.pdb: