Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLSDLRKFKAEGRKFSCLTCYDASMAKAMELAEIDTILIGDSLGMAIQGRDSTLPVTVEDMAYHTAAVRRGNQHALIMTDLPFMSYATLKD-ALQNAKTVMQAGAQMIKIEGGAWLSETVQVLTRNGVPVCVHLGLTPQSVHVFGGYKLQARTREAADQLIADCTAVVEAGAAVLLLECVPAQLGQEIAELFPNTPVIGIGAGNATDGQVLVVQDMLGLTFGRVARFVRNFMKEQSGETAILDAFKAFHAAVQDQSFPAKEHTFQVEL 1O68 Chain:A ((3-263)) LITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDSLGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAELMAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFA-----------K-AQALLNDAKAHDDAGAAVVLMECVLAELAKKVTETV-SCPTIGIGAGADCDGQVLVMHDMLGIFPGKTAKFVKNFMQ---GHDSVQAAVRAYVAEVKAKTFPAAEHIF----

General information: TITO was launched using: RESULT: Template: 1O68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1526 -130785 -85.70 -525.24 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -85.70 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_1O68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O68-query.scw PDB file : Tito_Scwrl_1O68.pdb: