Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLSDINQMNMTTANTARLLITCEDKPGIVQAVSSFLYHQGANITALDQYATEAQGGRYFMRVEFEL--DHLQSRKDALIQTFAANVAERYGMQWRLAFVNDIKKVGILVSKVDHALLELLWRHARGSLPCEITHVISNHEDLREAVENFGILFTVIKVTKDNKAEAYAQIHEMMQ--GNDLLVLARYMQILSEDFVSKWEMKIINIHHSFLPAFVGANPYKQAYEKGVKLIGATAHYVTADLDQGPIIEQDVERVSHDYNVEQLRELGEDVERNVLARAVKWHLEDRIIVDGNKTVVF 3LOU Chain:A ((13-291)) -------------------LTLSCPSAAGQVAAVVGLLDRHRCYVDELTVFDDDL-SARFFVRCVFHATD----LRVDALRREFEP-IAERFRMQWAIHDVAARPKVLIMVSKLEHCLADLLFRWKMGELKMDIVGIVSNHPDFAPLAAQHGLPFRHFPITADTKAQQEAQWLDVFETSGAELVILARYMQVLSPEASARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIEQVVERVDHSYRPEQLLAVGRDVECITLARAVKAFIERRVFLNGDRTVVF

General information: TITO was launched using: RESULT: Template: 3LOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1297 -101174 -78.01 -373.34 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -78.01 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_3LOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LOU-query.scw PDB file : Tito_Scwrl_3LOU.pdb: