Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAALHQPSQVQLNQDGNLKHFLTIEGLSKENLTKILDTAQSFLDDNNNLINRPLLEGRTVMNLFF-ENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPKV--AIINAGDGRHAHPTQAMLDMLTIRREMKKPFEDLSVAIIGDIKHSRVARSDVAALQTLGCKDIRVIAPNTLLPVGFSEYGDHVRLFNNMDEGITGCDVIIALRIQNERIDSPALSSQSEFYRMYGLNKERLSLAKPDCIVMHPGPMNRGVEIDSSIADGEQSVILKQVTNGIAVRMAVLALSMQGQLQEQGLIDAIAL 4BJH Chain:B ((32-319)) -------------------MRSLISSLDLTREEVEEILKYAKEFKEGKEETIKASAV-------LFFSEPSTRTRLSFEKAARELGIETYLVSGSESSTVKGESFFDTLKTFEGLGFDYVVFRVPFVFFPY---KEIVKSLNLRLVNAGDGTHQHPSQGLIDFFTIKEHFGE-VKDLRVLYVGDIKHSRVFRSGAPLLNMFGAK-IGVCGPKTLIPRDVEVF--KVDVFDDVDKGIDWADVVIWLRLQKERQKENYIPSESSYFKQFGLTKERFEKVK---LYMHPGPVNRNVDIDHELVYTEKSLIQEQVKNGIPVRKAIYKF-----------------

General information: TITO was launched using: RESULT: Template: 4BJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1576 -12464 -7.91 -43.73 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -7.91 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4BJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BJH-query.scw PDB file : Tito_Scwrl_4BJH.pdb: