Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKFMNGWVNGFIGVAIFAGSLPATRVAVTGFEPGFLTAARAVIAGVLGLILIFLLKEK-KPAKQDWWPLAIVALGVVVGFPLFTALALQYMNAAHSIVFVSLLPLATAIFAVVRGGEKPNLFFWIFAVLGSLVVFTYMFFLSGE----ASFGIGDLYMLIAIILCGFGYAEGGVLSKKIGGWQVICWALILALPIMLLATLFYMPVSFQDVSTSAVAGLVYVSLFSMLIGFFFWYKGLAQGGIAAISQLQLLQPLMGLAIAALLLHEHVSWSMLMVTAVTILCVAAAKKFA 5I20 Chain:E ((2-284)) --SRSSATLIGFTAILLWSTLALATS-STGAVPPFLLTALTFTIGGAVGIAAGLARGVGLSVLRQPWP-VWVHGIGGLFGYHFFYFSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGARAGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFAR-VPTEVVAGFCLATAALSALCHILFEPS---VWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLL-

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1362 -238419 -175.05 -857.62 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain E : 0.70 3D Compatibility (PKB) : -175.05 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: