Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQQYRLDELAHLVKGELIGEGSLQFSNLASLENAEVNHLTFVNGEKHLDQAKVSRAGAYIVTAALKEHLP-EKDNFIIVDNPYLAFAILTHVFDKKISSTGIESTARIHPSAVISETAYIGHYVVIGENCVVGDNTVIQSHTKLDDNVEVGKDCFIDSYVTITGSSKLRDRVRIH------------SSTVIGGEGFGFAPYQGKWHRIAQLGSVLIGNDVRIGSNCSIDRGALDNTILEDGVIIDNLVQIAHNVHIGSNTAIAAKCGIAGSTKIGKNCILAGACGVAGHLSIADNVTLTGMSMVTKNISEAGTYSSGTGLFENNHWKKTIVRLRQLADVPLTQITKRLDHIQAQIESLESTFNLRK 4IHG Chain:A ((12-341)) ------RLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVVMT---QDDLPFAKSAALVVKNPYLTYARMAQILD-----TTPQPAQNIAPSAVIDATAKLGNNVSIGAN------AVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIYHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDD-----MSKRLKSLE--------------

General information: TITO was launched using: RESULT: Template: 4IHG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1747 -83085 -47.56 -262.10 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -47.56 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4IHG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IHG-query.scw PDB file : Tito_Scwrl_4IHG.pdb: