Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLLQIAEPGQSSAPHQHRIAIGIDLGTTHSLAATVLSGKPKVLNDVQNRRLLPSIVHYG-DNTTHYGEEAKPFIIADPKNTIVSVKRFMGRSKADIKFQH-----PYELVGSEKNEMPAFETRAGRKTPVEISAEILKQLKDRAEDSLQNPVNGAVITVPAYFDEAQRQATRYAAQLAGLNILRLLNEPTAAAVAYGLDQESNLATDRNYVIYDLGGGTFDVSILRF----SQGVFEVLATGGHTALGGDDLD-RLI---VKWAKKQLNIDVLSDE-DYAVFIVAARQAKEQLST--QDSVELKLLEATLT--------LDRPTFESIIQVALDKTISVCKRVLRDAKLELTDIQNVVLVGGSTRSYAVQKAVREVFAQEPLCTINPDEVVAIGASITANQLIGNSQDGSLLLDVTPLSLGLETMGGLVERLISRNTAIPVARRQEFTTYQDGQTAMLIHVVQGERDLVEHCRSLGRFVLHGIPPMTAGQARIEVTFQVDADGLLTVSAREATSGVQAHIDIKPSYGLSEADTERLLIEGFQHAEEDKNLRHLKETKVEAERELEALEQALKVDADLLDEKQLEALNSAKESLKAQLEGSDIQAIEQAVQQLKVHSDAFAALRMNRHIDHALKGTKLDDWSKSN 4JN4 Chain:A ((5-587)) ---------------------IGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDA-WVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTG---NRTIAVYDLGGGAFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKD-VLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSM----GGAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQEL--------------------------------

General information: TITO was launched using: RESULT: Template: 4JN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3066 140324 45.77 251.48 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 45.77 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4JN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JN4-query.scw PDB file : Tito_Scwrl_4JN4.pdb: