Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTDPLVSCENLTSIVNLEHLMTTPVSFHPQAFIELALSRGVLKFGEFTLKSGRVSPYFFNAGLLNDGEALSLLAQGYADKLTQCE-NVDVIFGPAYKGIPFVAATAVALSQTHNKSVPWGFNRKEAKDHGEGGVLVGAAVEGKKVWIIDDVITAGTAIREVVTILKNAGATIAGVLVALDRQERGQGELSAIQEVQKELEIPVHALITMKDLMDYLEAKGEK-EALANMQAYREKYGI 3N2L Chain:A ((29-238)) ----------------------------YQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEFDVLFGPAYKGIPIATTTAVALADHHDVDTPYCFNRKE--------NLVGSKLEGR-VMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEKGKGELSAIQEVERDFGCAVISIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI

General information: TITO was launched using: RESULT: Template: 3N2L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 918 -61823 -67.34 -309.11 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -67.34 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.637

(partial model without unconserved sides chains): PDB file : Tito_3N2L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N2L-query.scw PDB file : Tito_Scwrl_3N2L.pdb: