Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAVFVDELDQVPDDSIVIFSAHGVSKAVQQEAERRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIGHEGHPEVEGTMGQYDKLKGGDIYLVEDEADVAALEVRHPEKLAFVTQTTLSIDDTAKVIDALRAKFPNIQGPRKDDICYATQNRQDAVRDLAEKCDVVLVVGSPNSSNSNRLRELAERMGKAAYLVDNADQLEQSWFNDTCKIGVTAGASAPEILIKQVIQRLQDWGAQAPKELEGREENITFSLPKELRIHVTQA 3ZGN Chain:A ((17-325)) MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGG-MYLVESPDDVWKLTVKNEEKLSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQLGGGEAIPLEGREENIVFEVPKELR------

General information: TITO was launched using: RESULT: Template: 3ZGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1790 -186314 -104.09 -602.96 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -104.09 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_3ZGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZGN-query.scw PDB file : Tito_Scwrl_3ZGN.pdb: