Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSICIFCGSSLGSNPIFQQIAQLTGEAIAKQGKTLVYGGGRSGLMGVVADSALQAGG--QVIGVIPRALV------------DRELAHPGLT---KLYVVENMHERKTKMADL------SDGFIALPGGAGTLEEIFEQWIWAQLGIHQKPCAFLNVAGFYEDLLKMIQGTVDNGFSQARFVDKLIASDKIEDILQQFEQYQAPAPKWTNADVQP 5AJU Chain:A ((62-261)) ---ICVFCGSSGGASPAHMEAARQLGRVMAENNIDLVYGGGTVGLMGEVARTVCSINGPESVHGIIPEALVRYERDGTYQTVKDNKQVVPTETVYGRTTVVKDMHTRKKMMAEEVISGGPGSGFIGLSGGYGTMEEVFEVITWNQLGIHTKGICLLNVEGYWDGILQWINMAAAQGFVQPGNETIVVSAGDAEGAVRALREYK-------------

General information: TITO was launched using: RESULT: Template: 5AJU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 840 -36650 -43.63 -207.06 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -43.63 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_5AJU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJU-query.scw PDB file : Tito_Scwrl_5AJU.pdb: