Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEVTAKLRGAAISAQKARLVADLIRGKSVAHALNILNFSNKKAAVLVKKALESAIANAEHNNSLDVDDLKVSTIYVDEGMSLKRIMPRAKGRADRITKRTCHITVKVGV 4CSU Chain:S ((1-108)) -METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSHITVVVS-

General information: TITO was launched using: RESULT: Template: 4CSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 440 -56916 -129.35 -527.00 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain S : 0.90 3D Compatibility (PKB) : -129.35 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_4CSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CSU-query.scw PDB file : Tito_Scwrl_4CSU.pdb: