Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQPKRTKFRKVHKGRNTGLAHRGSTVSFGSIAIKATERGRMTARQIEAARRAISRRIKRGGKIFIRVFPDKPITEKPLEVRMGNGKGNVEYWVCEIKPGKILYEIEGVNEDLAREAFALAAAKLPFKTTIVTRTVM 4CSU Chain:M ((1-136)) MLQPKRTKFRKMHKGRNRGLAQ-GTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM

General information: TITO was launched using: RESULT: Template: 4CSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 572 -34912 -61.03 -256.70 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain M : 0.96 3D Compatibility (PKB) : -61.03 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.668

(partial model without unconserved sides chains): PDB file : Tito_4CSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CSU-query.scw PDB file : Tito_Scwrl_4CSU.pdb: