Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNKQRWMGGVVLLGGGVLLAALLLKGNEEIKQVDVQPQTSTSPKLQAKPKQSAQEGQMVQLQPLAVDVETEKRLLEEQRRSREKAVAEQEARAAEFLAMQQQAEADAARKAAAEYAAINARRAAAQESSDNIPPEVAGSENKAKGQQTDTKKSVDLAKADADKKAAEAKRLAEADKKAAE-AKRQAEADKKAAEAKRQAE--ADKKAAEAKRQAEADKKAAEAKRKAEAEKKAEAEKARELLENGDKKWMVQVALAANQANADAVVSKLRAKGYKVTTSPTSKGIRIMVGPAKDRDTADTTRKKITSDASLNMKSAWVIDWVPLDQRKSD 4JN4 Chain:A ((476-593)) ---------------------------------------------------------------------------------------------------------------------------------ADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEA-KMQELAQVSQK-----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 173 27546 159.22 239.53 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 159.22 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_4JN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JN4-query.scw PDB file : Tito_Scwrl_4JN4.pdb: