Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKRLIGLFSPDLAIDLGTANTLIYAPGRGIILNEPTVVAIRHSGSQKIVAAVGLDAKQMLGRTPANISAIRPMKDGVIADFEVTETMLNQFIGKVHEKRLFPPAPRVVVCVPCKSTLVERRAIREAVFNASARDVRLIEEPMAAAIGAGMPVEQACGSMVVDVGGGTTEIAIISLQGCVYADSLRIGGDVFDEQIINYVRKAHGCVIGETTAEIIKKEVGMAV--SDGTTLEIEVRGRNLAEGVPRAITVTSDEITQAISDPLQSIVSAVKSALEQTPPELSSDIAERGIVLTGGGALLRNLDKLLAQETGLPVVVAEDPLTCVTRGGGKVLEFFDDPNHDMLFVG 4CZI Chain:A ((9-334)) ---------ISNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRN----KVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRK-GSGNPKVIVCVPS--TAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAP----GLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH------------

General information: TITO was launched using: RESULT: Template: 4CZI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1902 -82522 -43.39 -262.81 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -43.39 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_4CZI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZI-query.scw PDB file : Tito_Scwrl_4CZI.pdb: