Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLDTLQNKVVWITGASSGLGKALAGEFALQGAQVILTSRRFEELEEVRVGLLHPDQHLSVVADITNQEQVQEAYEQILKAKGRIDWLINNAGLSQRALIQDTTMATERAIMEVDYFSQVALTKTVLPTMLKQKSGRVVFVSSVAGLLGTQYRASYSAAKAAIHMWANSLRAEVADQGVEVSVIFPGFVKTNVSFNALNGAGQPQGHQDEAIENGLEADVFAEQSVKALMQGQEYIVVGGTKEKLGVMVSRMSPKLLYKMIRKTKVK 3U09 Chain:A ((4-200)) MSQFMNLEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGAQAISDYL--GDNGKGMALNVTNPESIEAVLKAITDEFGGVDILVNNADITRDNLLMRMKEEEWSDIMETNLTSIFRLSKAVLRGMMKKRQGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGFIETDMT-KALN--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3U09.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 970 -93391 -96.28 -474.07 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -96.28 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_3U09.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U09-query.scw PDB file : Tito_Scwrl_3U09.pdb: