Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNLTLSQFL---QQEKGNLTPELAQVIDTIAATCKTIDQALQKGALAGILGSAGNENVQGETQKKLDVISNDYLIDALKVHPHVGGLASEELDDFTPAQ--ENGEYLVLFDPLDGSSNIDINMCVGTIFSIL----PAKNAVTQAQDFMQAGTQQVAAGYVLYGPSTMMALTVGNGVAFFTLDPETQTFLLTTENVQVSADTQEFAINASNQRHWEQPVKQYIEELLAGKTSVREKDFNMRWVACMVGDVHRILCRGGIFLYPYDLKDPKKAGRLRLMYEANPMSMLIEQAGGASTTGRVRILEIEPTELHQRVPVIIGSKNEVERVTSYH 2OX3 Chain:A ((3-325)) ----TLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAGL-----------------QKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEHAKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTE-EDFLQPGNKQVAAGYVVYGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVKKYI-KFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPD--GKLRLLYECNPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERF-

General information: TITO was launched using: RESULT: Template: 2OX3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1708 -28852 -16.89 -97.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -16.89 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_2OX3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OX3-query.scw PDB file : Tito_Scwrl_2OX3.pdb: