Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAIYIHDVNFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTTFRLEPGERLAQYVLVPVVQAEFEQVEEFEETLRGAGGFGHTGKQ 2HRM Chain:A ((4-137)) -KIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAPGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWNRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFD---------------

General information: TITO was launched using: RESULT: Template: 2HRM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 571 -94197 -164.97 -702.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -164.97 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_2HRM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HRM-query.scw PDB file : Tito_Scwrl_2HRM.pdb: