Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAKEVAIQLTTHDALGAHARDEIGIHENTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLI-------MPSVLITGIAALAILGA-LSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL 3B9Y Chain:A ((141-250)) ------------------------------------------------------------------------------------------------------------------------------AFGAYFGLGVSIALTTAAQRAQPIESDATSDRFSMLGSMVLWLFWPSFATAIVPFEQMPQTIVNTL--LALCGATLATYFLSALFHKGKASIVDMANAALAGGVAIGSVCNI---

General information: TITO was launched using: RESULT: Template: 3B9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 -17032 -86.02 -166.98 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -86.02 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_3B9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B9Y-query.scw PDB file : Tito_Scwrl_3B9Y.pdb: