Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSSMSLTDYHATCPKAQKVKKGRNKFNASKIKLDGMTFDSTKEYKRYIELKALQQRGEIKELQHHTK-FELAPSVKI-EEEKRKK-----PALRYFADFTYFTAAGD--YVVEDVKSIATRKLPSYRNKKHLMKTVHNIDVREV 1T0F Chain:A ((58-138)) -----------------------------------VHHLLSDLELAVFLSL---EWESSVLDIREQFPLLPS-DTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAA-------------------------

General information: TITO was launched using: RESULT: Template: 1T0F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 177 -3769 -21.29 -52.35 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -21.29 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_1T0F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T0F-query.scw PDB file : Tito_Scwrl_1T0F.pdb: