Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVVDFSQLPPPNLIKELDFEQILAKRKERFISLFPTEQQLQWKEVLERESEPVTKLLEENAYMELILTNQINQDARALLLAFSSGSDLDHIAISYYGLKRLVITPANPNTVPPTAAVMETDENLKERCILSLSGLNTAGATNAYKFFAKSADGRVADASIISHEDNPCFLDVYITQHDSTNYEASPDLINIVQKALDPEDVRPVGDRPTVHSSK--AVPYQIQARLYISQTAENVMLLSVAKKRLEKYVENSKKIGQSIRRSAIYAALHVDGVSRVEILNLTSDIEISRSQHAFCTSLDIQIGGTE 3C4V Chain:A ((182-239)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGR----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 31 -1713 -55.24 -35.68 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -55.24 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_3C4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C4V-query.scw PDB file : Tito_Scwrl_3C4V.pdb: