Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFARQHFRKHQAKSAAEQAAEFGSMKNATAYELQLMQLNNDRARLKQIQSTENKIKLKAELLPTYAPYIDGILEAQSGVQDEILTEIMIWNIDTSNFSRALQIAEYVLVHNLALPDRFERTPACVITEEISAAFLKQLKTNVEID-IDVLKQLESLMTNPDLPEKTLDMPDQVKAKMYLALGKAEIRFISDKDKPDLVHTKAAADYLQKAVELDDKC-GGRGDLNMVQKLLDKFAPATEANKGDAAASS 2HR2 Chain:B ((20-150)) ------------------------------------------------------------------------------------------RQLVAGEYDEAAANCRRAMEISHTMPPEEAFDHAGFDAFCHAGLAEALAGLRSFDEALHSADKALHYFNR--RGEL-NQDEGKLWISAVYSRALALDGLGR---------GAEAMPEFKKVVEMIEERKGETPGKERMMEVAI-----------------

General information: TITO was launched using: RESULT: Template: 2HR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 510 -7049 -13.82 -54.64 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -13.82 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_2HR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HR2-query.scw PDB file : Tito_Scwrl_2HR2.pdb: