Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGIGLPFLGVIAIGVSKS-SGVYELAKR-INQTYAMVFTILLYLVIGPFFALPRLATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLAFLNPLGSVNQAPI-Q--PNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFA---LSENGGITLAQIADHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD 3OB6 Chain:B ((5-304)) --ADAHKVGLIPVTLMVSGNIMGSGVFLLPANLAS-TGGIAIYGWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLA--CWIGAIAMVVIGVGYLSYFFPILK-DPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALIPIVGIAVFGWFWFRGET-YMAAWNVSGLGTFGAIQSTLNVTLWSF---IGVESASVAAGVVKN-----PK---RNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDA----ARMALGDTAGAIVSFCAAAGCLGSLGGWTLLAGQTAKAA----------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3OB6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1506 -213589 -141.83 -731.47 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -141.83 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_3OB6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OB6-query.scw PDB file : Tito_Scwrl_3OB6.pdb: