Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEMAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHARVHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR 5ME1 Chain:O ((2-89)) ----SLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

General information: TITO was launched using: RESULT: Template: 5ME1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 240 -26394 -109.97 -299.93 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain O : 0.86 3D Compatibility (PKB) : -109.97 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.675

(partial model without unconserved sides chains): PDB file : Tito_5ME1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ME1-query.scw PDB file : Tito_Scwrl_5ME1.pdb: