Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYDVALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVH---AVG------------------------GTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASSKNE-EKVKKLGASAFAAYDKTNPG-------LQFADQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK 2EIH Chain:A ((1-260)) MRAVVMRARG-GPEVLEVADLPVPEPGPKEVRVRLKAAALNHLDVWVRKGVASP--KLPLPHVLGADGSGVVDAVGPGVEGFAPGDEVVINPGLSCGRCERCLAGEDNLCPRYQILGEHRHGTYAEYVVLPEANLAPKPKNLSFEEAAAIPLTFLTAWQMVVDKLGVRPGDDVLVMAAGSGVSVAAIQIAKLFGARVIATAGSEDKLRRAKALGADETVNY--THPDWPKEVRRLTGGKGADKVVDHT-GALYFEGVIKATANGGR------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1161 -21507 -18.52 -95.58 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -18.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_2EIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EIH-query.scw PDB file : Tito_Scwrl_2EIH.pdb: