Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSL--YEELLTHDNIVSAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEAVLFKGLGAKVIGYDIHQT-EAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR 1J4A Chain:A ((3-332)) ------KIFAYAIREDEKPFLKEWEDAHKDVEVEYTDKLLTPETVALAKGADGVVVYQQLDYIAETLQALADNGITKMSLRNVGVDNIDMAKAKELGFQITNVPVYSPNAIAEHAAIQAARILRQDKAMDEKVARHDLRW-APTIGREVRDQVVGVVGTGHIGQVFMQIMEGFGAKVITYDIFRNPELEKKGYYVDSLDDLYKQADVISLHVPDVPA-NVHMINDESIAKMKQDVVIVNVSRGPLVDTDAVIRGLDSGKIFGYAMDVYEGEVGIFNEDWEGKE-FPDARLADLIAR-PNVLVTPKTAFYTTHAVRNMVVKAFDNNLELVEGKEAETPVKV-------

General information: TITO was launched using: RESULT: Template: 1J4A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1667 -16827 -10.09 -51.46 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -10.09 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1J4A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J4A-query.scw PDB file : Tito_Scwrl_1J4A.pdb: