Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMFHCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFL-----NTGILLPLVKRIRKELP--DKDIWSWTGYTWEEMMLETPDKLE--LLSLIDILVDGRYDRTKRNLMLQFRGSSNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE 5EXJ Chain:A ((96-235)) ------------------------------------IGGDQCTRRCDFCQIDTGK----PAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWL-YAATVRAIKELNPSTGVE-LLIPDFNGEP---TRLAEVFESGPEVLAHNVETV-P-RIFKRIRP--AFTYRRSLGVLTAARDAGLVTK----------------

General information: TITO was launched using: RESULT: Template: 5EXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 598 -11273 -18.85 -86.05 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -18.85 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_5EXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EXJ-query.scw PDB file : Tito_Scwrl_5EXJ.pdb: