Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MTIMFVRKRNSALFTVILLGSILSGCQVVN---VKQQALNVTIANERNSILTQGKLSEASLNVLSMSGQEAKACTDSPDTCVNQLKNLPQILDEQLLSAASEMYLAKAMALSDSSECKISRFTKHKPTEEQKVIQNKYDECLDQQLSLLDKSIRYSYAYLFSTKRQPTDRIFDNRQVQIRDFYNQAIAKMVSVYDLRY--PQKNVVEPQ--IHIGKSVYSIDFEFHRQLSGQKLEKLISSYNLNFSGLRTINRRDGFGSEFVAVFPSSGKEDINEYILDPLNYSYKNGVNPNIHHARYLAATIVAEPKHAKTIQEIINDPEFVIRVYDPYRTDNINVAGKQYPLATNFSAPYGLWLAENNLGVAAYLSLIDRDQHLTMPHLYMLEPYNPNKKIIVLVH-GLASSPEAWIALTNDVMGDTVLRDNYQVWQVFYSTNMPILESRFQIYALLKQAFGSLNPSDPAAHDAVLVGHSMGGIISRLLVSDADITKPALEMMTIRQQNRFKKHPMVTERLQMHSISNFDRTIFLASPHRGTDYADRWFTLAARKIIRLP----GAFLSAVTTSLTTENLDIKDFLNNIDNGL----IQNGPSDLSHQSKFMELTENINPHQGLIFHSIMGNITKSDDPNVITDGIVPYKSAHLEGAKSEKVLPGGHSIQLTPQAVLELRRILREHLVEHGLYKP 4Z2T Chain:A ((11-591)) AETDVLIVGAGPAGAMSATLLASLGIRSLMINRWRSTSPGPRSHIINQRTMEILRDIGLEESAKSLAVPKEYMGEHVYATSLAGEEFGRIP-------------AWASHPQAHAEHELASPSRYCD--------LPQLYFEPMVVSEAALRGADVRFLTEYLGHVEDQ------DGVTARLLDHVSGAEYEVRAKYIIGADGAHSLVAQNAGLPFEGQMGGSINIEFSADLSSLCEHRKGDMYYMFVAALRMIRPWN----KWICVWGEITKEEAKKIIHEIIGTD---------------EIPVEVGPISTWTINQQYAVRNTSGRVFCMGDA--VHRHTPMGGLGLNTSVQDAYNLAWKLALVLKGTA------APTLLDSYDAERSPVAKQIVERAFKSLSTFPPVFEAL-----------------------SLPPAPTESEMAEALVRL---KDASEEGAKRRAALRKAMDATIIGLG------GGHGVELN-----QRYVSRAVFPDGTPDPGFVRDQEFFYQASTRPGAHLPHVWLTENQRRISTLDLCGKGRFTLLTGLSGAAWKHEAEQVSQSLGIELKVCVIGPGQEFVDTYGEYAKISE-IGESGALLVRPDMFIAFRAKDA--------------------------------SREGLEQLNVAVKSILGR------

General information: TITO was launched using: RESULT: Template: 4Z2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3210 39507 12.31 73.02 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.31 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.134

(partial model without unconserved sides chains): PDB file : Tito_4Z2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z2T-query.scw PDB file : Tito_Scwrl_4Z2T.pdb: