Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPLRLVSELDTALEFFIAKNAKGIVSISE------TEHSPMWSNTL-PENGCMSDFIRPEVQGKRSQDLPKFFRLNGSIYIYETLCLLEQSKI---------------FF--NENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 1H7H Chain:A ((3-242)) ------AVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGVAEVWVATDDPRVEQAVQAFGGKAIMTRNDHES-GTD---RLVEVM-HKV----EADIYINLQGDEPMIRPRDVETLLQGMRDDPALPVATLCHAISAAEAA-EPSTVKVVVNTRQDALYFSRSPIPYPRN-AEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEVA-ATGPGVDTPACLEKVRALMAQELA-

General information: TITO was launched using: RESULT: Template: 1H7H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1072 -66739 -62.26 -308.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -62.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1H7H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H7H-query.scw PDB file : Tito_Scwrl_1H7H.pdb: