Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYQMITREQCLYWDQEDELKKFKDEFALPEGVIYLDGNSLGARPKKSLAVAQHIISQEWGEDLINSW-NKADWWGLPTRLGDKVAKLVGAEQGEVVISDSTTLNLFKVLSAAVKIQADKFPEHKIIVAEKDAFPTDIYIIEGFIDLIQKGYQVELID-------GVEDLSRALE--KDVAVVVLSHVNYRTGYFYDMASINEQIHSKDALVIWDLCHSVGAVPMHLNQTDSDFAIGCTYKYLNGGPGSPALLWVNEKHRDQFWQPLSGWWSHKKPFDMAQHYEPANSIRRYLCGTQPVISMSLIECGVDIFLHADMQKIREKSLKLTDLFIQLVHQECSEFGFELITPLDHKHRGSHVSYRHEF--GYEIIQALIARGVIGDYRE----------------PAVLRFGITPLYLGFEDIWNAVQHLKATMLNSEWKNKEYLVRGEVT 4ISY Chain:C ((12-401)) --------------------------------SAYLDHAATTPMHPAAIEAMA-AVQRTIGNASS-LHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGA--HVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKFG-GPPGV-GALLLRRDVTC--VPLMHGGGQ----------------ERDIRSGTPDVASAVGMATAAQIAVD-GLEENSARLRLLRDRLVEGVLAEI--DDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSAPSHVLIAMGVDAASARGSLRLSLGH-TSVEADVDAALEVLPGAVARARRAALAAAG-----

General information: TITO was launched using: RESULT: Template: 4ISY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2130 -41083 -19.29 -115.73 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -19.29 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_4ISY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ISY-query.scw PDB file : Tito_Scwrl_4ISY.pdb: