Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVLVIMDPIETVNLKKDSTMAMLWAASRRGHELGYALQQDLYIDQGKAYGLISPLKVFEDYNHYYELGEKKKESIAAYDVVLMRKDPPFDMNFVYTTYVLEQAEREGSWIINKPQSLRDCNEKLFATQ-------FPE----LQVPTLVTSQQSLIREFITEH-GDVIVKPLDGMGGMGIFRLYQDGVNIGSTLEMLTELGTRPIMAQRYIPEIVEGDKRILMVNGEPIPYCLARIPQNGEVRGNLAAGGLGQARPLTENDKLIAAKVGPFLREKGLVFVGLDVIGN-----YVTEINVTSPTCIREIDAQFGTSIADDLFDVLEAGRPA 1I7L Chain:B ((4-307)) -VLLVVDEPHT----DWAKCFRGKKILGDYDIKVEQAEFSELNLVAHADGTYAVDMQV---LRNGTK-----VVRSFRPDFVLIRQHAFGMAENEDFRHLVIGMQYAGLPSINSLESIYNFCDKPWVFAQMVAIFKTLGGEKFPLIEQTYYPNHREM---LTLPTFPVVVKIGHAHSGMGKVKVENHYD-FQDIASVVALTQ-TYATAEPFIDAK--YDIRVQKIGNNYKA-YM-RTSISGNWKTNTGS-AMLEQIAMSDRYKLWVDACSEMFG--GLDICAVKAVHGKDGKDYIFEVMDCSMPLIGEHQVEDRQLITDLVISKMNQLL--

General information: TITO was launched using: RESULT: Template: 1I7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1380 -66736 -48.36 -232.53 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -48.36 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_1I7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I7L-query.scw PDB file : Tito_Scwrl_1I7L.pdb: