Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDP---KLI--AQADKIVFPGVGAMRDC--MQGMREAGIDEVVRK-AAFNKPVLAICVGMQALLQSSEENGGVDALGIFDGVVKHFPQMEGLKVPHMGWNQVHQMD---------------------------PSHPMWNNIEQ-DARFYFVHSYYVEPKDE-NLVAATCEYGVNFCTAI-HKDNLFATQFHPEKSH-TAGLQLLKNFVEWNI 3UOW Chain:A ((7-255)) YDKILVLNFGSQYFHLIVKRLNNIKIFSETKDYGVELKDIKDMNIKGVILSGGPYSVTEAGSPHLK----KEVFEYFLEKKIPIFGICYGMQEIAVQMNGE------------VKKSK------TSEYGCTDVNILRNDNINNITYCRNFSSAMDLYSNYKLMN--CCLFENIKSDITTVWMNHNDEVTKIPENFYLVSSSENC-LICSIYNKEYNIYGVQYHPEVYESLDGELMFYNFAY---

General information: TITO was launched using: RESULT: Template: 3UOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 751 -24604 -32.76 -139.00 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -32.76 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_3UOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UOW-query.scw PDB file : Tito_Scwrl_3UOW.pdb: